Fig 2: Analyses of ERG-associated genes in LnTE3a. The expression of ERG-associated genes including AR, PSA/KLK3 and PMEPA1 were analyzed by qPCR using TaqMan gene expression assays in LnTE3 cells (2.5 × 105) induced with doxycycline (dox, 1µg/ml). b. AR, PSA and PMEPA1 protein expression was analyzed by immunoblot in LnTE3 cells: Lane 1 (control) without dox, Lane 2 with dox, Lane 3 with dox and anti-miR-874 (20 nM) and Lane 4 with dox and pre-miR-449a (20nM). The relative quantification is indicated below. c. Corresponding mRNA expression was analyzed by qPCR. The modulation of miR-449a and miR-874 in LnTE3 cells was also separately verified as described. d. miR-449a expression was assayed in dox-treated LnTE3 cells incubated with pre-miR-449a (miR-449a, 20nM) and its negative control (miR-ctrl, 20nM) for 48 hr miR-specific TaqMan assay. e. Similarly, miR-874 expression was analyzed by TaqMan assays in dox-treated LnTE3 cells incubated with anti-miR-874 (20 nM) or respective negative control (anti-miR ctrl, 20nM) for 48hr.